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Hematology and Serum Chemistry Values for Winter Flounder (Pleuronectes americanus)

V.A. Dye1,2, T.C. Hrubec2*, J.L. Dunn1, S.A. Smith2
Volume 2, June 2001


Clinical analysis of blood to determine hematology and plasma biochemistry values is routinely used to assess the health of wild and domestic animals. Flounder culture is a fast growing segment of the U.S. aquaculture industry and tools are needed to monitor the health of these fish. This paper reports a complete hematologic and blood biochemistry profile for normal healthy winter flounder, Pleuronectes americanus, maintained in recirculated artificial seawater. The following hematologic values were determined: Packed cell volume, plasma protein, erythrocyte number, hemoglobin, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, and leukocyte, lymphocyte, neutrophil, monocyte, and thrombocyte numbers. A description of leukocyte morphology is presented. Additionally, the following serum biochemical values were determined: Total protein, albumin, globulin, sodium, potassium, chloride, calcium, phosphorus, magnesium, glucose, blood urea nitrogen, creatinine, total bilirubin, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, cholesterol and triglycerides. Analysis of blood parameters can enhance flounder culture by providing a means for the early detection and identification of infectious disease and of sub-lethal conditions affecting production performance.

1 Mystic Aquarium, 55 Coogan Boulevard, Mystic, CT 06355 USA

2 Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 USA

*Corresponding author, present address:
Center for Molecular Medicine and Infectious Diseases
1410 Prices Fork Rd
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute and State University
Blacksburg, VA 24061 USA